Job description
We are seeking a talented and motivated RNA Scientist to join our research and development team. The successful candidate will contribute to the design, development, and optimization of RNA vaccines aimed at reducing methane production in cattle, thereby supporting sustainable agriculture and environmental conservation efforts.
Responsibilities
- Synthesize RNA using in vitro transcription methods to generate RNA transcripts for various applications such as in vitro translation, transfection, and functional studies.
- Expertise in primer designing, cloning, expressing, and purifying recombinant proteins and antigens using molecular biology techniques.
- Develop and optimize expression systems by RNA transfection of several types of cell lines for high-yield production of antigens.
- Conduct molecular characterization and validation of expressed antigens.
- Develop and perform immunological assays to evaluate antigen-antibody interactions, such as ELISA, SPR, and FACS.
- Prepare and dispense solutions and chemicals for lab needs, including reagents, acids, bases, buffers, and stains.
- DNA (Sanger) and RNA sequencing including an understanding of sample preparation and sequencing platforms for accurate mutation identification and gene expression analysis.
- Collaborate with cross-functional teams to integrate antigen design and characterization into broader research and development projects.
- Work closely with bioinformaticians, immunologists, and other scientists to analyze data and derive meaningful insights.
- Maintain detailed records of experiments, protocols, and results.
- Prepare and present scientific findings internally and, when necessary, externally at conferences, scientific meetings, peer-reviewing manuscripts, and patent applications.
- Data Interpretation, analyze complex data sets, generate reports, and present findings to internal stakeholders and external partners.
- Contribute with management and collaborations with external resources CRO and research partners.
Qualifications
- Ph.D. in Molecular Biology, Veterinary Research, Immunology, Biochemistry, or related field, with a focus on RNA sciences with 2-3 years experience.
- Expertise and hands-on experience in RNA synthesis, transfection techniques, and quality control
- Proficiency in molecular biology techniques including hands-on protein expression, western blot, gel electrophoresis, qPCR, RT-PCR and purification methods.
- Hands-on experience in immunological assays and protein characterization techniques, such as ELISA, ELISpot SPR, MSD, and multicolor flow cytometry.
- Cultivate and propagate viable stock cultures of bacteria.
- Excellent data analysis, presentation, problem-solving skills, and written and oral communication skills
- Ability to work collaboratively in a team-oriented environment and cross-functionally
- Experience using Excel and Graphpad Prism and knowledge of statistics methods.
Good to Have
- A strong desire to stop near term climate change
We offer competitive compensation, excellent benefits, and opportunities for career advancement. Join us in our mission to make significant contributions to the field of vaccine development to mitigate methane emissions in ruminants.
The candidate should be authorized to work in the United States without visa sponsorship.
Equal Employment Opportunity
Pasture Biosciences is committed to providing equal opportunity and a work environment free of discrimination. All qualified applicants will receive consideration for employment without regard to race, color, religion, marital status, national origin, ancestry, sex, sexual orientation, gender identity, disability, medical condition, pregnancy, protected veteran status, age, citizenship, or any other characteristic protected by applicable laws.